Tissue folding at the organ–meristem boundary results in nuclear compression and chromatin compaction
نویسندگان
چکیده
Significance During development, growth deforms tissues and organs. This is notably the case during formation of new flowers in plants, as tissue folds young floral bud emergence. Here, we provide further evidence that organogenesis compresses cells at boundary, separating organ from stem cell niche, show this leads to nucleus compression chromatin changes. While mechanical forces are well known affect shape mammalian culture, demonstrates such an effect also occurs a developing organism suggests may help define boundary domains through large-scale effects.
منابع مشابه
Chromatin compaction at the mononucleosome level.
Using a previously described FRET technique, we measured the distance between the ends of DNA fragments on which nucleosomes were reconstituted from recombinant and native histones. This distance was analyzed in its dependence on the DNA fragment length, concentration of mono- and divalent counterions, presence of linker histone H1, and histone modifications. We found that the linker DNA arms d...
متن کاملNET23/STING Promotes Chromatin Compaction from the Nuclear Envelope
Changes in the peripheral distribution and amount of condensed chromatin are observed in a number of diseases linked to mutations in the lamin A protein of the nuclear envelope. We postulated that lamin A interactions with nuclear envelope transmembrane proteins (NETs) that affect chromatin structure might be altered in these diseases and so screened thirty-one NETs for those that promote chrom...
متن کاملThe nuclear pore as a chromatin boundary
he Cajal body (CB, formerly coiled body) is a prominent nuclear structure, yet its function remains obscure. Now, speculate that the CB is where small nuclear RNAs go for modification. Last year, Kiss's team identified a small guide RNA, U85, that directs both 2'-O-methylation and pseudouridylation of the spliceosomal component U5 snRNA. This year, they've identified six more RNAs, Novel guide ...
متن کاملEngineered Tissue Folding by Mechanical Compaction of the Mesenchyme.
Many tissues fold into complex shapes during development. Controlling this process in vitro would represent an important advance for tissue engineering. We use embryonic tissue explants, finite element modeling, and 3D cell-patterning techniques to show that mechanical compaction of the extracellular matrix during mesenchymal condensation is sufficient to drive tissue folding along programmed t...
متن کاملTissue Folding by Mechanical Compaction of the Mesenchyme
Department of Pharmaceutical Chemistry, University of California, San Francisco, CA, USA. Center for Cellular Construction, University of California, San Francisco, CA, USA. Graduate Program in Bioengineering, University of California, Berkeley, CA and University of California, San Francisco, CA, USA. Department of Orofacial Sciences, University of California, San Francisco, CA, USA. Foldscope ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences of the United States of America
سال: 2021
ISSN: ['1091-6490', '0027-8424']
DOI: https://doi.org/10.1073/pnas.2017859118